A multiplex liquidchip assay based on luminex xmap. Luminex based assay development stephen angeloni, ph. Currently we have hundreds of rna targets available in 330 plex assays using our using our quantigene plex reagent systems, with new targets being added weekly. This technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Introduction to xmap technology and milliplex assay panels. Procartaplex configurable simplex and multiplex immunoassay panels use luminex xmap technology to cover more than 400 cytokine, chemokine, growth factor, and other protein targets from human, mouse, rat, porcine, canine and nonhuman primate species. In addition to flowbased analyzers, magnetic beads can be read using the luminex magpix analyzer. Multiplex assays for the luminex instrument platform.
Novel formulation for eliminating nonspecific binding to. First, luminex uses proprietary techniques to internally color code microspheres with various fluorescent dyes. Luminex xmap technology is a beadbased multiplexed immunoassay system in a microplate format. Luminex corporation 12212 technology blvd austin, texas 78727. The microspheres can be coupled with reagents specific to a particular bioassay, such. Luminex has also launched and begun shipping its nxtag cov extended panel ruo, a unique multiplex panel that addresses the coronavirus pandemic using its existing beadbased nxtag technology. Comparison of the cervista hpv hr test and luminex xmap. Luminex is a method using polymerase chain reaction pcr in a commercial hpv genotyping kit yaneng bioscience shenzhen co. Featuring a flexible, openarchitecture design, xmap technology can be configured to perform a wide variety of protein or nucleic acid assays quickly, cost. System startup verify that the following procedures were performed when the magpix system was turned on for the first time. Luminex corporation owns 315 issued patents worldwide, including over 124 issued patents in the united states based on its multiplexing xmap platform. With over 10,000 instruments placed globally, the luminex platform is well recognized as the preferred multiplexing platform. Luminex instruments for xmap technology nonmagnetic magnetic figure 5. The magpix is a compact, robust, cost effective multiplexing system based on ccd imaging technology.
While other companies often specialize in either dna or protein analysis using different technology platforms, luminex is one of the few to offer the versatility of assaying a wide range of biomolecules and reactions on one platform. Perform assays more efficiently and cost effectively with luminex technologies with our unique approaches to biological testing, luminex is revolutionizing the industry by enabling companies and laboratories to perform bioassays more quickly and cost effectively, while ensuring optimum. Use our product documents resource to find product manuals, help guides, coq, msds and more. With multiplex pcr and luminex xmap technology, rasket kit enables detection of ras gene mutations with 48 kinds of amino acid substitutions simultaneously in single well. Featuring a flexible openarchitecture design, xmap technology can be configured to perform a wide variety of assays quickly, costeffectively, and accurately. Luminex xmap technology enables large numbers of biological tests to be conducted and analyzed quickly, costeffectively and accurately. All milliplex multiplex assays using luminex products. Bring your biomarkers to life with the most advanced instruments and software. Luminex xmap technology offers the versatility to perform a wide range of multiplexing assays from proteins to nucleic acids on a single platform, while other technologies often specialize in either dna or protein analysis using a variety of technology platforms. The luminex xmap technology is based on internally color coded microspheres also known as beads with red and infrared fluorescent dyes. Pdf xmap technology is applicable for highthroughput, multiplex and simultaneous detection.
Luminex xmap technology combines advanced fluidics, optics, and digital signal processing with fluorescently dyed microspheres to enable the quantitation of multiple nucleic acid or protein targets from a single sample. The dyes can be excited by a laser at a single wavelength to provide a distinct spectral signature to each of 100 bead types or regions. Sophisticated cell analysis doesnt have to be complicated or costly. The system can simultaneously detect many targets in a single sample up to 500, depending on system design. The luminex xmap technology offers significant advantages over traditional bioassays by offering highthroughput multiplexing of up to 100 analytes in a single well of a microtiter plate. Beads are read on a duallaser flowbased detection instrument, such as the luminex 200. As this technology matures, it will continue to improve and be incorporated into novel assays. Fluoroquinolone resistance detection in campylobacter coli. Luminex xmap technology overview clinical our xtag and multicodertx technologies serve a wide range of clinical applications, including infectious disease testing, providing exceptional sensitivity and specificity, unmatched throughput flexibility, and increased assay speed.
Featuring a flexible, openarchitecture design, xmap technology can be configured to perform a wide variety of protein or nucleic acid assays quickly, costeffectively, and. Luminex multicode technology, used for realtime polymerase chain reaction pcr and multiplexed pcr assays. Luminex switches are validated against many protocols and you can easily setup a combined network for your lighting, audio and video applications, without any worries or compromises. A magnet in the magpix analyzer captures and holds the magnetic beads in a monolayer, while two spectrally distinct lightemitting diodes leds illuminate the beads.
Luminexs xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Featuring a flexible, openarchitecture design, xmap technology can be configured to perform a wide variety of protein or nucleic acid assays quickly, costeffectively. The results of this study strongly support the potential of luminex xmap technology as an efficient molecular method for the rapid and accurate identification of c. Diphtheria, tetanus and pertussis using a luminex xmap technologybased pentaplex raissa nadege cabore 1, denis pierard 2 and kris huygen 1, 1 national reference centre bordetella and toxigenic corynebacteria, service immunology, scienti. Featuring a flexible, openarchitecture design, xmap technology can be configured. Luminex provides update on sarscov2 validation testing. The technology has been selected to be deployed for biobank profiling research in the human protein atlas hpa project. The investor relations website contains information about luminex corps business for stockholders, potential investors, and financial analysts. Beadbased multiplexing of up to 500 analytes per sample the most trusted multiplexing technology luminex s xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. We utilized onestep multiplex reverse transcriptionpcr rtpcr and luminex xmap technology to develop a respiratory multiplex liquidchip assay rmla for simultaneous detection of 6 common respiratory viruses, including influenza virus type a flua. This flexible technology is centered on the use of luminex xmap microspheres. In the late 1990s scientists at luminex invented xmap technology, a major. These instruments are available for the detection of cytokines, hormones, antigen, and antibodies in nhp body fluids such as serum, plasma, saliva, cerebrospinal fluid, vaginal and rectal swabs, gingival crevicular fluids, and tissue.
Feb 03, 2017 luminex xmap technology makes up the basis of luminexs core business. Advanced detection instruments the luminex 100 200 bio. This technique involves 100 distinctly coloured bead sets created by the use of two fluorescent dyes at distinct ratios. Featuring a flexible openarchitecture design, xmap technology can be configured to. Detection targets of interest are quantified on a luminex instrument.
Applications of luminex xmap technology for rapid, high. Procedures are presented as stepbystep instructions. Luminex xmap technology, the basis for our milliplex map assay kits, is a powerful platform for multiplex detection of proteins in a single biological sample. Advanced detection instruments the luminex 100200 bio. Luminex xmap technology, based on flow cytometry, employs tiny colored beads. Luminex technology southwest national primate research. Nov 29, 2017 principle of multiplex bead based immunoassay. How xmap technology works thermo fisher scientific us. Luminex s xmap technology combines advanced fluidics, optics, and digital signal processing with proprietary microsphere technology to deliver multiplexed assay capabilities. Read more research our xmap technology and its flexibl. The icl has two luminex with xmap multianalyte platform technology systems. Luminex multiplexing technology consists of beadbased immunoassays using xmap beads, high plex multiplexing instrument, and powerful software. Hardware the luminex 200 system includes the following hardware.
The muse cell analyzer packs 3parameter analysis into a compact, easy to use benchtop device, making flow cytometry accessible to anyone, anytime. The microspheres used in the luminex system have different spectral addresses color codes. Luminex xmap technology brochure coefficient of variation. Our assay kits can be run on any of these luminex systemsmagpix, luminex 100200, bioplex, or flexmap 3d. While other companies often specialize in either dna or protein analysis using different technology platforms, luminex is one of the few to offer the versatility of assaying a wide range of.
Microspherebased multiplex immunoassays scitech connect. The sample is added to a mixture of colorcoded beads, precoated with analytespecific capture antibodies. Luminex xmap microparticle technology xmap technology by luminex utilizes colorcoded microparticles that incorporate different proportions of two dyes. The procedures are presented as stepbystep instructions and assume basic knowledge of xponent software and xmap technology. Luminex xmap technology brochure free download as pdf file. Our procarta human, mouse and rat assays can quantitatively measure cytokines and chemokines. Jan 25, 2017 xmap technology is applicable for highthroughput, multiplex and simultaneous detection of different analytes within a single complex sample.
Luminex 200 system analyzer, luminex software, computer, monitor, keyboard, mouse, luminex xy platform instrument luminex xyp, luminex sheath delivery system luminex sd, barcode reader, sheath and waste containers, and xmap technology reagents. Highthroughput luminex xmap assay for simultaneous detection of antibodies against. Luminex 200 system user manual the xmap microspheres are not gently vortex the plate or fully suspended. Introduction to luminex xmap technology and applications for. The manuals text and figures offer examples when necessary.
The united states headquarters for luminex is in austin, texas and additional offices. Our milliplex portfolio of immunoassays is the largest portfolio of multiplex biomarker assays, based on luminex xmap technology, offering you consistent, highquality results, so that you can do your best work while saving time, labor and cost. A number of labs in the far east, europe and north america have already validated this panel and are planning to go live with it this week. Luminex xmap technology, the trusted, widely used platform for biomarker. A belgian serosurveillanceseroprevalence study of diphtheria. With luminex switches and the rlinkx protocol you dont have to worry about losing signal. Feature introduction to luminex xmap technology and. As a bead based detection system, luminex allows for simultaneous measurement of analytes in as little as 25 bl deriving maximum data from minimal sample input. While most of the simple western assays are for cell lysate analysis, the luminex technology provides complementary assays on cytokine, metabolite, immune response, serumplasma biomarker analysis etc. Milliplex multiplex assays using luminex life science. Pdf and excel export compatible automatic calculation of minmaxdc import data in exceltext formats from.
Converged networking is much more costeffective and timesaving. The luminex r xmap technology x danalyte, map multianalyte pro. One led identifies the analyte that is being detected and, the second led. Luminex was incorporated in 1995 and began commercial production of its first generation system in 1997. Luminex xmap technology enables scientists to measure multiple proteins in a single well.